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            <TitleText>Méthodes d'analyse en milieu marin</TitleText>
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          <TitleText>Les marqueurs biologiques des effets de polluants : l'acétylcholinestérase</TitleText>
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        <BiographicalNote>François Galgani est docteur es sciences, chef de projet à l’Ifremer Bastia, responsable des groupes européens en soutien à la directive cadre « Stratégie du milieu marin » (DCSMM).</BiographicalNote>
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        <Text language="fre">&lt;p&gt;Les strat&amp;eacute;gies de surveillance de la qualit&amp;eacute; de l&amp;#39;environnement littoral sont d&amp;#39;actualit&amp;eacute; pour des raisons &amp;agrave; la fois &amp;eacute;cologiques et socio-&amp;eacute;conomiques. Ce fascicule traite de la mesure de l&amp;#39;activit&amp;eacute; ac&amp;eacute;tylcholinest&amp;eacute;rasique chez les esp&amp;egrave;ces marines et de son inhibition en tant que marqueur d&amp;#39;effets neurotoxiques li&amp;eacute;s &amp;agrave; certaines familles de x&amp;eacute;nobiotiques et, notamment, aux pesticides. Cette application &amp;agrave; des fins de diagnostic n&amp;eacute;cessite &amp;agrave; la fois une connaissance approfondie de la cin&amp;eacute;tique de l&amp;#39;enzyme et de sa variabilit&amp;eacute; naturelle et une caract&amp;eacute;risation stricte des conditions d&amp;#39;extraction, de conservation des &amp;eacute;chantillons et de mesure de l&amp;#39;activit&amp;eacute;. Ces diff&amp;eacute;rents aspects sont d&amp;eacute;crits en d&amp;eacute;tails. La m&amp;eacute;thode de mesure de l&amp;#39;activit&amp;eacute; de l&amp;#39;ac&amp;eacute;tylcholinest&amp;eacute;rase telle qu&amp;#39;elle est d&amp;eacute;crite dans ce fascicule a &amp;eacute;t&amp;eacute; publi&amp;eacute;e par le CIEM (Conseil international pour l&amp;#39;exploration de la mer).&lt;/p&gt;</Text>
        <Text language="eng">&lt;p&gt;Strategies to monitor the quality of the coastal environment are a topical issue, for both ecological and socio-economic reasons. This booklet deals with measuring acetylcholinesterasic activity in various marine species and its inhibition as a biomarker of neurotoxic effects of certain families of contaminants, especially pesticides. Using it for environmental diagnoses requires both thorough knowledge of the enzyme&amp;#39;s kinetics and its natural variability, as well as strict characterisation of the conditions for extraction, sample storage and measuring the activity. These aspects are described in detail. The method to measure acetylcholinesterase activity described in this document was published by ICES (International Council for the Exploration of the Sea) in 1997. It is a benchmark method in the field.&lt;/p&gt;</Text>
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        <Text>Ce fascicule traite de la mesure de l'activité acétylcholinestérasique chez les espèces marines et de son inhibition en tant que marqueur d'effets neurotoxiques liés à certaines familles de xénobiotiques et, notamment, aux pesticides. </Text>
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